Marine Chemistry Laboratory

Sampling Procedures

Sampling Procedures

Sampling Procedures

The Marine Chemistry Laboratory at the School of Oceanography provides marine and freshwater analytical services to the University and oceanographic communities. We are an accredited laboratory with the State of Washington. The lab specializes in the analyses of nutrients, total N and total P, salinity, chlorophyll, oxygen, DOC in aqueous samples, and OC in particulate samples. Prices and other analyses are listed below:

ANALYSIS HOMEBASE OPERATIONS SHIPBOARD ANALYSES

Salinity

Analyses are run on Guildline models 8400B and 8410 Portasal - Calibration is with IAPSO Standard Seawater

Analyses are run on Guildline models 8400B and 8410 Portasal - Calibration is with IAPSO Standard Seawater

Oxygen

Analyses are run using the Carpenter (modified Winkler) with a Metrohm 765 Dosimat buret

Analyses are run using the Carpenter (modified Winkler) with a Metrohm 765 Dosimat buret

Nutrients (phosphate, silicate, nitrate, nitrite, & ammonia)

Analyses and calibration follow the protocols of the WOCE Hydrographic Program using a Technicon AAII system

Analyses and calibration follow the protocols of the WOCE Hydrographic Program using a Technicon AAII system

Chlorophyll a and phaeopigments

Analysis is acetone extraction and fluorometric detection on a Turner Designs TD-700 fluorometer

 

Dissolved Organic Carbon 

 

(aqueous samples)

Analysis is with a Shimadzu TOC-Vcsh DOC analyzer

 

Total Organic Carbon, Nitrogen, and Hydrogen (solid samples)

Analysis is by Exeter Analytical CE-440 CHN analyzer

 

Oceanography chemical analyses price list

Nutrients: (PO4, Si(OH)4, NO3, NO2, NH3) all ions included in base price

$14.50 each

$24.00 each if dilution required

Syringe filters for samples: $2.25 each

Total N&P

$17.00 each

 

Chlorophyll a

$12.00 each

Dissolved Organic Carbon (DOC)

Filtered liquid sample $22.50 each

C clean filters $.75 each

C clean sample vial $2.00 each

Particulate Carbon and Nitrogen (CHN)

On 25 mm filters $37.00 each

Sediment samples: $46.50 each

C clean filters $.75 each

Salinity

$17.00 each

TSS

$13.80 each

Turbidity

$6.90 each

Oxygen titrations

$15.00 each

1L suite of the six oxygen reagents: $460.00

Important notes:

  • Add 15.6% to the total cost for any work done not charged to a UW budget.
  • Supplies are not always available on short notice, so plan ahead and give them at least one full week's notice when requesting filters.
  • Logsheets must be provided with the samples. They should include budget numbers, sample identification numbers, sampling locations, filtration volumes (where appropriate), and whether the sample contains fresh or marine water.
  • Business is very good in the lab, so short turnaround time for sample analyses must be discussed with Kathy or Aaron ahead of time.
  • Clients will be charged for any shipping costs associated with supplies and coolers
  • We will not accept or process any samples fixed with HgCl, or Azide
  • C14  labelled samples are not allowed in the building, no exceptions.

 

Accreditation Codes and Detection Limits

Analysis Method References EPA/SM# NELAC Code Detection_Limits
PO4 UNESCO(1994) EPA 365.5_1.4_1997 WM920270 0.03uM
SiOH4 UNESCO(1994) EPA 366 WM920240 0.76uM 
NO3 UNESCO(1994) EPA 353.4_2_199 10068209 0.08uM
NO2 UNESCO(1994) EPA 353.4_2_1997 10068209 0.01uM
NH4 UNESCO(1994) EPA 349 WM920220 0.07uM
         
Total N Valderrama(1981) SM 4500-P J WM920270 1.08uM
Total P Valderrama(1981) SM 4500-P J WM920270 0.04uM
         
Salinity UNESCO(1994) SM 2520 B-93 20040055 0.002PSU
         
Ckhlorophyll_a UNESCO(1994) EPA 445 WM100080 0.02ug/L
         
DOC UNESCO(1994) SM 5310 B-00 20137819 100ug/L
         
POC UNESCO(1994) EPA 365.5_1.4_1997 10081206 10ug
PN UNESCO(1994) EPA 365.5_1.4_1997 10081206 1ug
Dissolved O2 UNESCO(1994) EPA 360.2 50001600 0.5uM kg-1
         

UNESCO (1994). Protocols for the joint global ocean flux study (JGOFS) core measurements. Vol. 29.
Valderrama (1981). The simultaneous analysis of total nitrogen and total phosphorus on natural waters. Mar.Chem (10), 109-122.
 


Please contact Katherine Krogslund (manager, kkrog@uw.edu) and/or Aaron Morello (aaronm@ocean.washington.edu) PRIOR to sampling to discuss your project/sampling plans.

Address: University of Washington, School of Oceanography, Marine Chemistry Lab, 1492 NE Boat St., Seattle, WA 98105
Map: Ocean Sciences Bldg., Room 346
Phone: (206) 543-9235 Fax: (206) 685-3354

Financial Particulars:

IRS/TaxID/Entity Number: 91-6001537

Cage Code: 0MCA1 (first character is a zero)

DUNS Number: 618134977

Make payments to:  University of Washington

Mail payments to:

University of Washington

Invoice Receivables

PO Box 94224

Seattle, WA 98124